Journal: Oncotarget

Article Title: The core sequence of PIF competes for insulin/amyloid β in insulin degrading enzyme: potential treatment for Alzheimer's disease

doi: 10.18632/oncotarget.26057

Figure Lengend Snippet: (A) Representative Western Blots of Aβ and IDE in neuronal cells after APP transfection. (B) sPIF reduces Aβ formation in IDE dependent manner. (C) Identified regions of structural similarity within the set of protein structures by STRALCP. Clustering of structurally conserved fragments ( left ). STRALCP identified structural similarity between selected “ reference ” structure and other analyzed structures, represented as colored bars, based on C alpha - C alpha distance deviation at each position between the reference (top bar) and other structures ( right ). The colors indicate RMSD between aligned residues, ranging from green (below 2Å), yellow (below 4Å), orange (below 6Å), to red (above 6Å). STRALCP: STRucture ALignment-based Clustering of Proteins; RMSD: Room mean square distances; Aβ: Amyloid Beta; IDE: Insulin degrading enzyme; APP: Amyloid precursor protein; sPIF: synthetic PreImplantation Factor; * p<0.05; ** p<0.01; *** p<0.001 (ANOVA followed by two tail t test). In-vitro experiment results represent at least three independent experiments.

Article Snippet: We purchased plasmids containing amyloid precursor protein (APP) from Addgene (pCAX APP 695 #30137).

Techniques: Western Blot, Transfection, In Vitro